Here’s a tutorial from Corsolutions, a company based in Ithaca, New York that is utilized by academic, biotechnology, pharmaceutical and government researchers in seeking to enhance their microfluidic workflows, that discusses the process in fabricating a microfluidic device from PDMS. PDMS refers to polydimethylsiloxane, a silicon-based organic polymer that is widely used for microfluidic devices because it is easy to work with, biocompatible, economical and transparent down to 280 nanometer.
- First, mix the PDMS two-part kit, which is composed of the base and the cross-linking agent;
- Next, pour the liquid pre-polymer over a previously fabricated master mold.
- Cure the PDMS in an oven at 65°C for 24 hours.
- Peel the PDMS device from the master mold and cut as desired.
- Next the inlet and outlet ports on the device need to be fabricated.
- Fnally to interface the PDMS microdevice with fluid delivery pumps and detectors, CorSolutions compression connectors can be quickly and non-permanently placed over the inlet and outlet ports to make leak-tight connections.